airway epithelial cell growth medium  (PromoCell)

Journal: bioRxiv

Article Title: SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types

doi: 10.64898/2026.02.05.703986

Figure Lengend Snippet: (A) Schematic of the experimental design for induction of systemic senescence with doxorubicin and sample collection across time points. (B) Cluster identities assigned to doxorubicin-treated lung samples profiled by snRNA-seq. (C) UMAP projection of lung samples colored by cluster identity. (D) UMAP projections of senescent cells in lung samples across time points. (E) Bar plot showing the number of senescent cells from lung per cluster and condition. (F,G) GSEA plots of gene set association scores for p53, EMT, NF-κB, Apoptosis, and Hypoxia hallmark pathways (F) and senescence signature lists SenePy, SeneSig, SenMayo, hUSI (G); in fibroblast, epithelial, and endothelial clusters on Day 6. (H) Schematic of the analysis pipeline applied to published aging lung snRNA-seq datasets. (I) UMAP projection of aging lung samples showing senescent cell distribution by age group. (J) Schematic of ligand-receptor inference analysis between senescent fibroblasts and non-senescent epithelial cells. (K) Chord diagram displaying ligand-receptor interactions between senescent fibroblasts (sender cells) and non-senescent epithelial cells (receiver cells) inferred through CellPhoneDB. (L) Dot plot showing expression of the specified ligands across fibroblast clusters at 23 months of age. (M) Schematic and GSEA plot explaining and displaying TGFβ signaling pathway increase during aging in non-senescent epithelial cells at 23 months versus 3 months. See also Figures S11-13 .

Article Snippet: HSAEC lung epithelial cells (ATCC, PCS-301-010) were cultured using an Airway Epithelial Cell Basal Medium plus Bronchial Epithelial Cell Growth Kit (ATCC).

Techniques: Expressing

Journal: bioRxiv

Article Title: A host ATPase essential for rhinovirus replication is an antiviral target with a high barrier to resistance

doi: 10.64898/2026.05.13.723454

Figure Lengend Snippet: (A-C) CB-6644 antiviral assays in cell lines. (A) HeLa-H1 cells, (B) BEAS-2B cells or (C) HeLa-E8 cells were infected with the indicated RV types (MOI 20 for A , MOI 1 for B-C ). Cells were treated at 1 hpi with DMSO or the indicated concentrations of CB-6644. Viral titres were quantified at the indicated times post-infection. N=4 or 5 independent experiments. (D-G) CB-6644 antiviral assays in WD-PNECs. (D) Workflow for generation of WD-PNEC cultures. Primary nasal epithelial cells (PNECs) were sampled via nasal brushing from volunteers, expanded in monolayers, and seeded into Transwells. When 100% confluent, after 4-8 days of incubation, apical medium was removed to initiate air-liquid interface (ALI), which triggers cell differentiation and the formation of a pseudostratified epithelium containing ciliated epithelial cells, goblet cells and basal cells. After 28 days of incubation, high quality WD-PNEC cultures were infected apically with the indicated RV (MOI 0.01). CB-6644 or DMSO was added apically 16 h before (E) or at different time points after (F) infection, as indicated. Viral titres in apical washes collected at the indicated times were quantified. N= 3 (E, RV-A16 and RV-B14) or 2 (E, RV-C15 and F) independent donors. (G) Viability of WD-PNECs apically treated with 2 μM CB-6644 or DMSO for 192 h, or with 1% Triton X-100 (TX100) for 2 h, presented as percentage viability relative to DMSO-treated control. N= 3 independent donors. For panels A-C and G, data are shown as individual points, coded by shape according to experimental replicate, with means. For panels E-F, data are shown as means (± SD) connected by lines colour-coded by treatment. Statistical tests: two-tailed paired t -test (A-C), one-way ANOVA with Dunnett’s post-hoc test (G). **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant. See also Figure S2.

Article Snippet: After sampling (5 turns within 1 nostril), nasal brushes were placed inside 15 ml conical tubes and washed with Airway Epithelial Cell Growth Medium (C-21160, Promocell) supplemented with 1X Airway Epithelial Cell Growth Medium supplement pack (C-39160, Promocell) and 1% (v/v) penicillin/streptomycin (15140122, Thermo Fisher Scientific) (monolayer medium).

Techniques: Infection, Incubation, Cell Differentiation, Control, Two Tailed Test